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Life sciences · Journal article
Current Biochemistry · October 22, 2026
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Obesity is a metabolic disorder characterized by a body mass index (BMI) >30 and is associated with an increased risk of degenerative diseases such as diabetes mellitus type 2, stroke, and cardiovascular disease. Anti-obesity drugs such as orlistat are effective; however, they may cause side effects, highlighting the need for natural alternatives. This study aimed to evaluate the effect of a combination of 70% ethanol extracts of srikaya leaves (Annona squamosa L.) and kelor leaves (Moringa oleifera Lam) based on phytochemical content, antioxidant capacity, and pancreatic lipase inhibitory activity. Extraction was carried out using a sonication method, followed by formulation of extract combinations and evaluation of total phenolic content (TPC), total flavonoid content (TFC), antioxidant capacity using DPPH and FRAP methods, and lipase inhibition. The results showed that the single extract of A. squamosa leaves exhibited the highest activity, with TPC of 95.15 mg GAE/g, TFC of 47.57 mg QE/g, DPPH of 395.10 mg TE/g, FRAP of 476.30 mg TE/g, and lipase inhibition of 64.15%. The extract combination showed synergistic effects on DPPH activity and pancreatic lipase inhibition, but tended to be antagonistic for TPC, TFC, and FRAP.