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Life sciences · Journal article
Journal of Global Antimicrobial Resistance · August 1, 2026
Early or partial results. Treat as a signal, not a conclusion.
This is a descriptive genomic characterization of a multidrug-resistant E. coli ST410 strain carrying bla NDM-5, integrated with a retrospective survey of global prevalence patterns in public sequence databases. The study documents genetic relatedness, plasmid structure, and geographic distribution but does not measure clinical outcomes or test interventions, making it primarily useful for epidemiological surveillance and hypothesis generation rather than practice guidance.
Single-strain whole-genome sequencing with retrospective bioinformatic analysis of public genome database. One paediatric patient (10 months old) with E. coli EC6030 infection; global dataset of 7,606 bla NDM-5-carrying E. coli sequences from NCBI RefSeq, predominantly clinical specimens, mostly from Asia.. Intervention: None; observational genomic characterization.. Compared with: Comparison to 7,606 previously sequenced bla NDM-5-carrying E. coli genomes in NCBI RefSeq database; closest relative identified post-hoc.. Index case location not specified; global database analysis from NCBI; closest relative from United Kingdom (2020)..
EC6030 (ST410, phylogroup C) was resistant to β-lactams, carbapenems, fluoroquinolones, trimethoprim-sulfamethoxazole, chloramphenicol, and tetracycline; susceptible to amikacin and gentamicin. The bla NDM-5 gene was located on a 46,161 bp IncX3 plasmid flanked by multiple insertion sequences with high similarity to previously reported bla NDM-carrying plasmids. The closest global relative to EC6030 is ST410 strain P24_WM1_05.20 from the United Kingdom (2020), differing by 61 SNPs.
No quantification of prevalence trends, temporal changes, or mortality/morbidity outcomes.
These findings support strengthened genomic surveillance and infection control strategies for CRE dissemination but do not provide evidence to change antimicrobial prescribing, diagnostic algorithms, or clinical management protocols. The data support continued monitoring for clonal and plasmid-mediated spread of this lineage.
A single-strain genomic characterization study with retrospective database analysis of prevalence patterns; descriptive epidemiology without clinical outcomes or intervention trials to guide practice.
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These findings support strengthened genomic surveillance and infection control strategies for CRE dissemination but do not provide evidence to change antimicrobial prescribing, diagnostic algorithms, or clinical management protocols. The data support continued monitoring for clonal and plasmid-mediated spread of this lineage.
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Objectives Carbapenem-resistant Enterobacteriaceae (CRE) pose a major public health threat due to extensive drug resistance and nosocomial spread. This study characterized the genome sequence of a multidrug-resistant E. coli strain EC6030 carrying bla NDM-5 recovered from a 10-month-old patient, and investigated its relationship to global bla NDM-5 -positive E. coli. Methods Whole-genome sequencing was performed to analyze antimicrobial resistance genes, virulence determinants, plasmid content, and the genetic context of bla NDM-5 in EC6030. A total of 7,606 bla NDM-5 -carrying E. coli genomes were retrieved from the NCBI RefSeq database. Core-genome multilocus sequence typing, minimum-spanning tree analysis, and SNP-based phylogenetic analysis were used to assess global distribution and genetic relatedness. Results EC6030 was resistant to multiple antimicrobial classes, including β-lactams, carbapenems, fluoroquinolones, trimethoprim-sulfamethoxazole, chloramphenicol, and tetracycline, but remained susceptible to amikacin and gentamicin. Genomic analysis assigned EC6030 to ST410 and phylogroup C. The bla NDM-5 gene was located on a 46,161 bp IncX3 plasmid flanked by multiple insertion sequences. This plasmid showed high sequence similarity to previously reported bla NDM -carrying plasmids among E. coli and K. pneumoniae isolates in the public database. Globally, bla NDM-5 -carrying E. coli strains are most prevalent in Asia, primarily from clinical specimens, and occur sporadically. The closest relative to EC6030 is a ST410 strain P24_WM1_05.20, recovered from the United Kingdom in 2020 which differing by 61 SNPs. Conclusion These findings highlight the clinical significance and potential clonal and plasmid-mediated dissemination of bla NDM-5 -carrying E. coli ST410 lineage and support strengthened genomic surveillance, antimicrobial stewardship, and infection control strategies globally.
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