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High-throughput Screening Assays · Journal article
Talanta · July 8, 2026
Early or partial results. Treat as a signal, not a conclusion.
This paper describes a validated high-throughput UHPLC-MS/MS method for simultaneous quantification of 230 environmental pollutants in serum, optimized via phospholipid removal purification. Analytical performance meets standards (RSD <15.1%, 98.3% recovery within 80–120%). Exploratory analysis of serum samples from a single cohort identified associations between specific pollutants (PFHpA, PFOA, dehydroacetic acid) and hyperuricemia, but these are observational findings without established causality or external validation.
Methods development with exploratory cross-sectional cohort analysis. Serum samples from Fuqing cohort participants; detailed inclusion/exclusion criteria and demographics not provided. Intervention: Analytical method: UHPLC-MS/MS with phospholipid removal purification for simultaneous quantification of 230 environmental pollutants. Compared with: Comparative evaluation of sample pretreatment techniques (low-temperature concentration, liquid-liquid extraction, direct protein precipitation); PRP selected as optimal. Fuqing cohort (China implied); specific study centres not stated.
Method achieved simultaneous quantification of 230 environmental pollutants in serum 98.3% of compounds exhibited satisfactory recoveries (80–120%); relative standard deviation <15.1% 22 contaminants detected with detection frequencies >30% in Fuqing cohort participants
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This method enables high-throughput screening for exposome-wide associations in population studies. The identified associations with hyperuricemia are preliminary and should be validated in independent cohorts with appropriate adjustment for confounders before informing clinical practice.
This is a methods development study with an observational application in a single cohort; the analytical validation is sound, but the epidemiological findings are exploratory associations without a comparator or causal design.
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This method enables high-throughput screening for exposome-wide associations in population studies. The identified associations with hyperuricemia are preliminary and should be validated in independent cohorts with appropriate adjustment for confounders before informing clinical practice.
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Simultaneous determination of environmental pollutants with complex compositions and wide-ranging acid dissociation constants in biological matrices presents a significant analytical challenge. This study developed a highly sensitive and broad-spectrum analytical method based on ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). By employing phospholipid removal purification (PRP) technology and optimizing mobile phase pH conditions, we achieved simultaneous quantification of 230 environmental pollutants in serum. Comparative evaluation of sample pretreatment techniques revealed that low-temperature concentration caused preservative loss, while liquid-liquid extraction showed poor recovery for multiple compound classes. The PRP method was selected as the optimal approach due to its superior performance in reducing matrix effects compared to direct protein precipitation. Method validation demonstrated accurate quantification of all target analytes (relative standard deviation, RSD <15.1%), with 98.3% of compounds exhibiting satisfactory recoveries (80-120%). Analysis of serum samples from the Fuqing cohort participants detected 22 contaminants with detection frequencies >30%, among which PFHpA and PFOA were identified as risk factors for hyperuricemia. Notably, we provide the first evidence of a dose-response relationship between dehydroacetic acid exposure and hyperuricemia prevalence in a human population. This study established a robust solution for simultaneous screening of exposome-scale pollutants in complex biological samples.
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