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Life sciences · Journal article
Diagnostic and Interventional Radiology · September 14, 2026
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following exposure to Cre recombinase. This pictorial essay illustrates the technical methodology for liver tumor induction in Oncopigs and demonstrates the angiographic use of tumor-bearing animals for evaluation of transarterial therapies. Liver tumors were induced using an ex vivo transgene activation approach in which liver biopsy specimens were incubated with an adenoviral vector expressing Cre recombinase and injected percutaneously into the liver under ultrasound guidance. Tumor development occurred in 34/37 (92%) induction sites across 17 Oncopigs and was confirmed by contrast-enhanced computed tomography. Hepatic arteriography using standard clinical devices enabled selective catheterization of tumor-feeding arteries. This pictorial essay provides a technical guide for investigators studying liver-directed therapies in a translational large-animal model.